Polymerase Chain Reaction: Theory and TechnologyPaperback: ISBN 978 1 912530 24 3 EAN 9781912530243 This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. Topics covered include: principles of PCR, fluorescent chemistries, instrumentation, quantification strategies, extraction and purification of nucleic acids, sample preparation, controls for validation, primers and probes, standardization of methods, MIQE guidelines, mRNA expression and